Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis.
- Author:
Bin YANG
1
;
Min-tong XIN
;
Dong-mei LU
;
Qiao-ling QU
;
Hua LI
;
Lu-qi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzaldehydes; analysis; Catechin; analysis; Catechols; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; standards; Electrochemistry; Fagopyrum; chemistry; Gallic Acid; analysis; Hydroxybenzoates; analysis; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(12):1171-1174
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.
METHODThe HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.
RESULTThe HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and ( - ) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.
CONCLUSIONThe method can be used to control the quality of Rhizoma fagopyri dibotoryis.
