Chemical constituents from branch of Macaranga adenantha and their TNF-alpha inhibitory activity.
- Author:
Ming MA
1
;
Xiao-ya SHANG
;
Su-juan WANG
;
Shuai LI
;
Yong-chun YANG
;
Jian-gong SHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; drug effects; Coumarins; chemistry; isolation & purification; pharmacology; Ellagic Acid; analogs & derivatives; chemistry; isolation & purification; pharmacology; Euphorbiaceae; chemistry; Macrophages, Peritoneal; cytology; drug effects; secretion; Mice; Plant Stems; chemistry; Plants, Medicinal; chemistry; Tumor Necrosis Factor-alpha; metabolism
- From: China Journal of Chinese Materia Medica 2007;32(12):1175-1179
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the chemical constituents of the brach of Macaranga adenantha, and evaluate their TNF-alpha inhibitory activity.
METHODThe chemical conshifuents were isolated and purified by chromatographic methods. Structures of the compounds were identified by spectroscopic methods. The TNF-alpha secretion inhibitory activity of the mouse peritoneal macrophages was evaluated by MTT methods.
RESULTTen compounds were isolated and their structures were identified as: cleomiscosin A (1), cleomiscosin B (2), ellagic acid 4-O-alpha-D-rhamnopyranside (3), ellagic acid 4-O-beta-D-xylopyranoside (4), vanillic acid (5), (24R) -stigmast-4-en-3-one (6), (24R) -stigmast-3, 6-dione (7), (24R) -6beta-hydroxy-stigmast-4-en-3-one (8), daucosterol (9), beta-sitosteryl glucoside-6'-O-heptadecoicate (10). At a concentration of 10 micromol x L(-1), compounds 1, 3 and 4 showed inhibitory activity to TNF-alpha secretion of the mouse peritoneal macrophages with the inhibitory rates of 57.0%, 64.4%, and 57. 4%, respectively.
CONCLUSIONAll compounds were isolated from genus Macaranga for the first time. Compounds 1, 3, and 4 were active against TNF-alpha secretion of the mouse peritoneal macrophages.
