Determination of three glycosides from herbs of Swertia punicea by RP-HPLC.
- Author:
Xiu-qiao ZHANG
1
;
Feng-jiao HUANG
;
Jiao-chun CHEN
;
Yan-wen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; standards; Glucosides; analysis; Glycosides; analysis; Iridoid Glucosides; Iridoids; analysis; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Swertia; chemistry; Xanthones; analysis
- From: China Journal of Chinese Materia Medica 2007;32(12):1184-1186
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a RP-HPLC method for determination of three glycosides in Swertia punicea.
METHODChromatographic column: Alltimal C18 (4.6 mm x 250 mm, 5 microm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL x min(-1). Wavelength: 254 nm. Column temperture: 30 degrees C.
RESULTThe calibration curves of gentiopicroside, mangiferin and swertrianolin were in good linearity over the range of 31.3-281.7, 0.31-2.78, 0.55-4.91 microg, (r = 0.9996, 0.9993, 0.9995). The average recoveries were 103.36%, 101.42% and 97.39%, with RSD less then 3% (n = 5).
CONCLUSIONIt is a simple and sensitive meathod in controlling the quality of S. punicea.
