Effects of micrometer compound rhizoma coptidis on nuclear factor-kappaB and inflammatory factors in rabbit fed with high lipid diet.
- Author:
Bin WANG
1
;
Si-ming GUAN
;
Xi-dong LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; drug effects; metabolism; C-Reactive Protein; metabolism; Coptis; chemistry; Diet, Atherogenic; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Enzyme-Linked Immunosorbent Assay; Inflammation Mediators; blood; Interleukin-1; blood; Male; Microspheres; NF-kappa B; genetics; metabolism; Particle Size; Plants, Medicinal; chemistry; Powders; RNA, Messenger; genetics; metabolism; Rabbits; Random Allocation; Reverse Transcriptase Polymerase Chain Reaction; Tumor Necrosis Factor-alpha; blood
- From: China Journal of Chinese Materia Medica 2007;32(12):1207-1210
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of the micrometer compound rhizoma coptidis on inflammatory factors and its possible mechanism in rabbit fed with high-lipid food.
METHODThe levels of CRP, IL-1 and TNF-alpha were all determinated by ELISA method. The mRNA and activity of NF-kappaB were determinated by RT-PCR and EMSA, respectively.
RESULTThe level of CRP, IL-1 and TNF-alpha were significantly increased by feeding for 16 weeks with high-lipid diet in rabbit. It was significantly increased that the mRNA and the binging activity with DNA of NF-kappaB in thorax aorta of rabbits fed by high-lipid diet, too. The micrometer compound rhizoma coptidis can reverse the effects of high-lipid diet on CRP, IL-1, TNF-alpha and NF-kappaB.
CONCLUSIONThe micrometer compound rhizoma coptidis can inhibit the expression of inflammatory factor possibly through inhibitting the expression and activity of NF-kappaB.
