Effect of different light intensity on Polygonum cuspidatum callus.
- Author:
Tao WEN
1
;
Li LIANG
;
Yang ZENG
;
Xiao YU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Fallopia japonica; chemistry; growth & development; radiation effects; Light; Plant Leaves; chemistry; growth & development; radiation effects; Plant Stems; chemistry; growth & development; radiation effects; Plants, Medicinal; chemistry; growth & development; radiation effects; Stilbenes; analysis; Tissue Culture Techniques; methods
- From: China Journal of Chinese Materia Medica 2007;32(13):1277-1280
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of light intensity (LI) on the callus induction and resveratrol content of Polygonum cuspidatum.
METHODTaking leaves and stem segment of P. cuspidatum as explants, the callus was inducted and cultured under different LI. The growth status of callus was observed and analyzed. The content of resveratrol was determined by HPLC.
RESULTThe formation and growth of the stem segment callus were optimal under low light treatment (1340-1560 lx). The resveratrol content was 18.350 microg x g(-1) in fresh stem segment and 140.074 microg x g(-1) in dry stem segment, which was two times higher than that of wild P. cuspidatum.
CONCLUSIONIntroduction and status of P. cuspidatum callus were obvious diverse under different LI, the resveratrol content of P. cuspidatum was enhanced under appropriate LI.
