Effect of PMTG on atherosclerotic lesion formation and expression of ICAM-1 and VCAM-1 in ApoE-deficient mice.
- Author:
Wei FANG
1
;
Hui-xin ZHANG
;
Lu-ya WANG
;
Yan-wen QIN
;
Ying WU
;
Wei WANG
;
Bin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antioxidants; pharmacology; Aorta, Thoracic; drug effects; metabolism; pathology; Apolipoproteins E; deficiency; genetics; Atherosclerosis; blood; pathology; prevention & control; Cholesterol; blood; Cholesterol, LDL; blood; Endothelial Cells; drug effects; pathology; ultrastructure; Female; Glycosides; isolation & purification; pharmacology; Immunohistochemistry; Intercellular Adhesion Molecule-1; biosynthesis; Malondialdehyde; blood; Mice; Mice, Knockout; Microscopy, Electron, Transmission; NF-kappa B; metabolism; Nitric Oxide; blood; Plants, Medicinal; chemistry; Polygonum; chemistry; Random Allocation; Triglycerides; blood; Vascular Cell Adhesion Molecule-1; biosynthesis
- From: China Journal of Chinese Materia Medica 2007;32(13):1320-1323
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protecting effect of polygoni multiflori total glycosides (PMTG) on the atherosclerotic lesion formation and the expression of ICAM-1, VCAM-1 in aolipoprotein (apo) E-deficient transgenic mice.
METHODThirty-two female apoE-deficienct mice were randomized into four groups: PMTG high dose group (150 mg x kg x d), low dose group (25 mg x kg x d), atorvastatin positive control group (5 mg x kg x d), and model group. At the end of the tenth week, all mice were killed. The serum levels of Total cholesterol (TC), Triglyceride (TG), High-density lipoprotein-cholesterol (LDL-C) were measured by enzyme dynamics method. Transmission electron microscopy (TEM) were used to observe the morphologic changes of aortic endothelia cell. The expressions of NF-kappaB were studied by SABC immunohistochemistry.
RESULTAs compared with the model control group. (1) PMTG could reduce the levels of serum TC, TG significantly (P < 0.01), and LDL-C level significantly (P < 0.01). (2) It could increase the levels of serum NO and the anti-oxidation capacities significantly (P < 0.01), but reduce the levels of serum MDA significantly (P < 0.01). (3) PMTG could keep the normal morphology of aortic endothelial cell. (4) PMTG could deregulated the expression of NF-kappaB in aortic wall.
CONCLUSIONPMTG could inhibit the occurrence and development of atherosclerotic lesions by its anti-oxidation abilities, which reduce LDL-C level. The low LDL-C level could deregulated the of expression of NF-kappaB, which could deregulated ICAM-1 and VCAM-1 in AopE-/-mice in aortic wall through.
