Protective and antioxidant properties of wasp (Vespa magnifica) honeycomb extract: a potential inhibitor against acidified ethanol-induced gastric lesions.
- Author:
Xue-qing XU
1
;
Le YU
;
Shu-wen LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antioxidants; pharmacology; therapeutic use; Ethanol; adverse effects; Female; Honey; Male; Materia Medica; pharmacology; therapeutic use; Neutrophil Infiltration; drug effects; Oxidative Stress; drug effects; Peroxidase; metabolism; Rats; Rats, Wistar; Stomach Ulcer; chemically induced; prevention & control; Wasps; chemistry
- From: Journal of Southern Medical University 2011;31(7):1107-1110
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo examine the protective effects of wasp (Vespa magnifica) honeycomb extract (WCE) against gastric lesions in rats induced by 60% acidified ethanol, and evaluate its capacity to suppress oxidative stress in the gastric tissue.
METHODSWistar rats were subjected to intragastric administration of 60% acidified ethanol to induce gastric lesions following an 8-day oral pretreatment with WCE at 0, 25, 100 and 150 mg/kg or with saline. The levels of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, myeloperoxidase (MPO) activity and total antioxidant capacity in the gastric tissues were determined.
RESULTSOral administration of 25, 100 and 150 mg/kg WCE prior to 60% acidified ethanol administration significantly inhibited the formation of gastric lesions (with a reduction by 44.2%-87.1%), decreased the mucosal MPO activity (by 16.4%-56.6%) and increased the total antioxidant capacity of the gastric tissue (by 0.5, 1.47 and 1.83 folds, respectively) in a dose-dependent manner. At a high concentration (above 1 mg/ml), WCE also exhibited a stronger DPPH radical scavenging activity than butylated hydroxytoluene (BHT).
CONCLUSIONThe ethanol extract of wasp honeycombs can suppress the formation of acidified ethanol-induced gastric lesions by reducing free radical oxidation and neutrophils infiltration in the gastric tissue in rats.