Construction of a PCR chip for detecting genes associated with metastatic colorectal cancer and its preliminary application.
- Author:
Xiao-qin XIAO
1
;
Na TANG
;
Chao LIU
;
Hong-xia WANG
;
Qiang JIANG
;
Yong-jian DENG
;
Yan-qing DING
Author Information
- Publication Type:Journal Article
- MeSH: Colorectal Neoplasms; genetics; pathology; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Neoplasm Metastasis; diagnosis; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; methods; Prognosis
- From: Journal of Southern Medical University 2011;31(7):1169-1174
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a PCR chip with a gene panel for predicting and diagnosing metastatic colorectal cancer.
METHODSThe PCR chip was constructed by integrating 29 genes related to colorectal cancer metastasis identified by gene chip analysis and 3 housekeeping genes into a gene panel. The PCR chip was used for detecting the mRNA expressions of the integrated genes in colorectal cell lines, cancerous specimen and adjacent normal mucosa. The primers for amplification were refined and optimized by several rounds of preliminary reactions.
RESULTSThe PCR chip containing the 29 candidate genes and 3 housekeeping genes was successfully constructed, which showed specific amplifications of the genes. The results of the PCR chip for detecting the mRNA of the 29 genes related to colorectal cancer metastasis showed a concordance rate of 86% (25 out of 29) with the gene chip data. Application of the PCR chip in the examination of the clinical specimens identified 15 differentially expressed genes between metastatic colorectal cancer and colorectal cancer without metastasis.
CONCLUSIONThe constructed PCR chip is reliable in the prediction of metastasis of colorectal cancer, and provides a molecular means for evaluating the prognosis of colorectal cancer metastasis.
