25-hydroxyvitamin D3-induced increases of normal human airway epithelial cell permeability is not mediated by upregulated ZO-1 expression.
- Author:
Xiao-ting ZHOU
1
;
Hai-jin ZHAO
;
Shao-xi CAI
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; metabolism; Calcifediol; pharmacokinetics; pharmacology; Cell Line; Cell Membrane Permeability; drug effects; Epithelial Cells; cytology; metabolism; Humans; RNA, Messenger; genetics; metabolism; Zonula Occludens-1 Protein; genetics; metabolism
- From: Journal of Southern Medical University 2011;31(7):1187-1189
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of 25-hydroxyvitamin D3 on the permeability and ZO-1 expression in normal human airway epithelial cells.
METHODSMTT assay was used to assess the viability of human airway epithelial cell line 16HBE following a 24-hour exposure to different concentrations of 25-hydroxy vitamin D3, and the transepithelial electrical resistance (TER) of the cell monolayer was measured using a Millicell-ERS voltohmmeter. Real-time quantitative RT-PCR was employed to determine the changes of ZO-1 mRNA expression in the cells following the exposures.
RESULTSExposure to 25-hydroxyvitamin D3 resulted in significantly increased permeability of 16HBE cells, but the exspression of ZO-1 showed no obvious changes. 25-hydroxyvitamin D3 at 4×10(-9) mol/L showed the strongest effect in increasing the permeability of cell monolayer.
CONCLUSION25-hydroxyvitamin D3 increases the permeability of normal bronchial airway epithelial cell monolayer in vitro, but this effect is not mediated by upregulation of ZO-1 expression.
