Feasibility of peptide mass fingerprinting for differential diagnosis of IgA and non-IgA nephropathy.
- Author:
Jing GAO
1
;
Yong WANG
;
Xin-yu WEN
;
Hong-hao LU
;
Zhen-nan DONG
;
Ya-ping TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Diagnosis, Differential; Feasibility Studies; Female; Glomerulonephritis, IGA; diagnosis; Humans; Kidney Diseases; diagnosis; Male; Middle Aged; Peptide Mapping; methods; Peptides; chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; methods; Young Adult
- From: Journal of Southern Medical University 2011;31(8):1309-1313
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility of peptide mass fingerprinting for non-invasive differential diagnosis of IgA nephropathy (IgAN) from the non-IgA nephropathy (IgAN).?
METHODSAccording to the results of renal biopsy, 56 patients were divided into IgAN group (n=28) and non-IgAN group (n=28), and peptide mass fingerprints were acquired from these patients using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
RESULTSNine different peptides were identified between IgAN and non-IgAN. The two most distinctive differentially expressed peptides, with peptide peak values of 4476.46 and 1968.10, showed area under curve values of 86.18% and 79.77%. Principal component analysis demonstrated that the accumulated explained variance of the first 8 differential peptides reached 95%, suggesting the feasibility of differential diagnosis of IgAN from non-IgAN. Comparison with the Matrix protein database identified the peptide with a relative molecular mass of 5338.08 as a fragment of mucin 4 inform and the 2082.77 peptide as fragment of α1-II type collagen inform.
CONCLUSIONMALDI-TOF MS is feasible for differential diagnosis of IgAN and non-IgAN and also has great potentials in the classification of the subtypes of other systemic diseases.