Effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on human gastric cancer cells and its mechanism.
- Author:
Jian-fa CHEN
1
;
Yin-xiang CHEN
;
Ping LI
;
Ming FU
;
Ying-nian LV
;
Li LI
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Diterpenes; isolation & purification; pharmacology; Humans; Proto-Oncogene Proteins c-bcl-2; metabolism; Pteris; chemistry; Stomach Neoplasms; pathology; bcl-2-Associated X Protein; metabolism
- From: Journal of Southern Medical University 2011;31(8):1345-1348
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis-inducing effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F), a compound isolated from Pteris semipinnata L (PsL), on human gastric cancer SGC7901 cells and explore its mechanism.
METHODSThe inhibitory effect of 5F on SGC7901 cells was observed by MTT assay and flow cytometry, and the changes of the expression of Bcl-2 and Bax in SGC7901 cells following 5F exposure were evaluated by Western blot analysis.
RESULTS5F inhibited the proliferation of SGC7901 cells in a concentration- and time-dependent manner, and the cell apoptosis induced by 5F was confirmed by Annexin V-EGFP staining and caspase-3 activation assay. The cell apoptosis induced by 5F was associated with decreased Bcl-2 and increased Bax expressions.
CONCLUSION5F exposure induces apoptosis in SGC7901 cells by activating mitochondrial apoptotic pathways.
