Role of interleukin-17 and Th17 cells in acute renal allograft rejection in mice.
- Author:
Zhong-zhou SI
1
;
Ting LI
;
Jie-qun LI
;
Hai-zhi QI
;
Xu-biao XIE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Graft Rejection; blood; immunology; Interleukin-17; blood; Kidney Transplantation; adverse effects; immunology; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Th17 Cells; immunology; Transplantation, Homologous
- From: Journal of Southern Medical University 2011;31(8):1374-1377
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of Thl7 cells and the cytokine interleukin-17 (IL-17) in acute allograft rejection in mice.
METHODSMouse models of kidney transplantation were randomly divided into rejection group and isograft group. On the post-operative day (POD) 3 and 7, we tested the serum IL-17 level using enzyme-linked immunosorbent assay and measured the number of Th17 cells in the renal grafts by flow cytometry. The grafts were harvested and fixed in 10% formalin to prepare paraffin sections for routine pathological inspection.
RESULTSCompared to isograft group, the allograft group showed a significantly higher level of serum IL-17 on POD3 and POD7 (P<0.05), and the level of IL-17 is significantly higher on POD7 than on POD3 (P<0.05). The allograft group showed more infiltrating Th17 cells in the grafts on POD3 and POD7 (P<0.05), and the cell number was significantly greater on POD7 (P<0.05). Pathological examination also showed an increased severity of graft rejection with the post-transplantation time.
CONCLUSIONThl7 cells may play an important role in the development of renal graft rejection. IL-17 may serve as a potential specific indicator for predicting allograft rejection.
