On-line detection of hydrocortisone concentration in rat subcutaneous adipose with liquid chromatography-mass spectrometry/mass spectrometry.
- Author:
Li-zhen HUANG
1
;
Lin WANG
;
Yuan LIU
;
Lu-qi XIONG
;
Guo-feng LI
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Cutaneous; Animals; Chromatography, Liquid; methods; Hydrocortisone; administration & dosage; pharmacokinetics; Male; Microdialysis; methods; Rats; Rats, Wistar; Subcutaneous Fat; chemistry; metabolism; Tandem Mass Spectrometry; methods
- From: Journal of Southern Medical University 2011;31(8):1387-1391
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method for detecting the topical concentration of hydrocortisone (HC) in the subcutaneous adipose of rats using microdialysis sampling technique and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS).
METHODSTopical samples were collected by applying the probe into the subcutaneous adipose of rats, with alcohol (5%)-ringers solution as the perfusion solution. A LC-MS/MS method was established for detecting the HC concentration in the dialysates.
RESULTSThe protonated precursor to produce ion transitions monitored for HC was m/z 363.2→121.1. The calibration curve was linear over the range of 0.5-1000 ng/ml, with the intra- and inter-day precision and accuracy within ∓15%, and no significant matrix effect was noted. The in vivo recovery of the probe was about 59%.
CONCLUSIONA selective and sensitive method has been successfully established for the on-line HC detection in the subcutaneous adipose of rats.
