Determination of silver content in silver-loaded coral hydroxyapatite in relation to the biocompatibility.
- Author:
Yu ZHANG
1
;
Qing-shui YIN
;
Yu ZHANG
;
Jian LI
;
Xu-qiong CHEN
;
Dong FU
Author Information
- Publication Type:Journal Article
- MeSH: 3T3 Cells; Animals; Anthozoa; chemistry; Anti-Bacterial Agents; analysis; pharmacology; Biocompatible Materials; chemistry; pharmacology; Bone Substitutes; chemistry; pharmacology; Cells, Cultured; Durapatite; chemistry; pharmacology; Materials Testing; Mice; Silver; analysis; chemistry; pharmacology
- From: Journal of Southern Medical University 2011;31(8):1411-1415
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the amount of silver in silver-loaded coral hydroxyapatite (Ag(+)-CHA) bone substitute and its impact on the biocompatibility of this material with mouse embryonic osteoblast cells.
METHODSAg(+)-CHA was prepared by immersing coral hydroxyapatite in a serial concentration of silver nitrate solutions. The amount of silver in the prepared Ag(+)-CHA was measured by inductively coupled plasma atomic emission spectrometry (ICP-AES). The viability of MC3T3-E1 cells incubated with Ag(+)-CHA was measured by MTT colorimetric assay, and the cell growth and morphological changes were observed by inverted phase-contrast microscope and confocal laser scanning microscope.
RESULTSThe amount of silver loading in the bone substitutes prepared by immersion in 1×10(-2), 1×10(-3), 5×10(-4), 10(-4), 8×10(-5), and 5×10(-5) mol/L silver nitrate solutions were 4127.67∓47.35, 167.90∓11.00, 83.42∓4.51, 30.20∓2.32, 22.39∓4.09, and 15.11∓0.55 µg/g, respectively. A low silver content in the material (prepared with silver nitrate solution of less than 8×10(-5) mol/L) showed no significant inhibitory effect on the growth of MC3T3-E1 cells or produced noticeable cytotoxic effect. On the materials prepared with 8×10(-5) and 10(-5) mol/L silver nitrate solution, the osteoblasts displayed active proliferation similar to those incubated on materials without silver loading. The confluent cells showed a normal fusiform morphology with tight arrangement.
CONCLUSIONAg(+)-CHA with low silver content has a good biocompability and can promote the proliferation and growth of MC3T3-E1 cells in vitro, suggesting the clinical potential of this material as a anti-infection bone substitute.