Activation of Rho-kinase pathway is involved in angiotensin II-induced contraction of human airway smooth muscle cells.
- Author:
Ning LI
1
;
Ying MENG
;
Yuan-xiong CHENG
;
Yi NIU
;
Bin SHEN
;
Xu LI
;
Wen-yan LAI
Author Information
- Publication Type:Journal Article
- MeSH: Amides; pharmacology; Angiotensin II; pharmacology; Asthma; physiopathology; Biphenyl Compounds; pharmacology; Bronchi; cytology; Humans; Muscle Contraction; drug effects; Muscle, Smooth; cytology; Primary Cell Culture; Pyridines; pharmacology; Signal Transduction; drug effects; Tetrazoles; pharmacology; rho-Associated Kinases; metabolism
- From: Journal of Southern Medical University 2011;31(8):1416-1420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate of the regulatory effect of Rho-kinase pathway activation on angiotensin II (Ang II)-induced contraction of human airway smooth muscle cells (HASMCs) in vitro.
METHODSCultured primary HASMCs were divided into control group, AngII group, AngII + irbesartan group and AngII + Y-27632 group with corresponding treatment. AngII-induced contraction of HASMCs was evaluated using collagen gel lattices and observed morphologically using immunofluorescence assay. Western Blotting was significantly performed to examine the protein expression of Rho-kinase signal pathway.
RESULTSAngII-induced HASMC contraction was inhibited by treatments with irbesartan and Y-27632 as shown by gel contraction assay (P<0.001). Y-27632 treatment produced a stronger inhibitory effect than irbesartan on the expression of phosphorylated moesin, a substrate of Rho kinase (P<0.05).
CONCLUSIONAngII induces the contraction of HASMCs partially as a result of activation of Rho-kinase pathway.
