Ultracytochemical observation of the intracellular localization of H+-adenosine triphosphatase.
- Author:
Shen-qiu LUO
1
;
Zhi-yong KE
;
Yan-meng LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Membrane; enzymology; Hepatocytes; cytology; enzymology; ultrastructure; Histocytochemistry; methods; Kidney; cytology; enzymology; ultrastructure; Lysosomes; enzymology; Male; Organelles; enzymology; Rats; Rats, Wistar; Vacuolar Proton-Translocating ATPases; metabolism
- From: Journal of Southern Medical University 2011;31(8):1431-1433
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the ultracytochemical localization of H(+)-adenosine triphosphatase (H(+)-ATPase) in the cell organelles.
METHODSThe localization of H(+)-ATPase in the cell organelles was observed in the hepatocytes and renal cells of Wistar rats using routine ultracytochemical methods.
RESULTSH(+)-ATPase activities were observed on the lysosomal membrane and nuclear envelope of the hepatocytes and proximal tubule epithelial cells of the nephron in Wistar rats.
CONCLUSIONThis finding supports the hypothesis that H(+)-ATPase (V-ATPase) is present on the plasma membrane and in the endomembrane system.
