Application of quantum dots fluorescent probes in tissue of human tongue squamous cell carcinoma.
- Author:
Jian-jiang ZHAO
1
,
2
;
Yu-hua HUANG
;
Zhi-ping WANG
;
Jie PAN
;
Jun CHEN@
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; Fluorescent Dyes; Humans; Quantum Dots; Tongue Neoplasms; Tumor Suppressor Protein p53
- From: West China Journal of Stomatology 2011;29(2):175-178
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study if quantum dots fluorescent probes can be applied to detect P53 protein and Bcl-2 protein in tissue of human tongue squamous cell carcinoma.
METHODSBy indirect immunofluorescence assay the same particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein respectively. Different particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein simultaneously in paraffin-embedded tissue section of human tongue squamous cell carcinoma under fluorescent microscope.
RESULTSP53 protein and Bcl-2 protein can be combined with quantum dots fluorescent probes and specific fluorescene can be observed with ultraviolet light excited. P53 protein was mainly distributed in the nucleus, and Bcl-2 protein major in the cytoplasm. P53 protein and Bcl-2 protein can be combined with different particle size quantum dots fluorescent probes respectively in the same paraffin-embedded tissue section of human tongue squamous cell carcinoma and two kinds of fluorescene can be observed.
CONCLUSIONQuantum dots fluorescent probes can be applied to detect two kinds of specific protein in paraffin-embedded tissue section of human tongue squamous cell carcinoma.