Expression of glucose transporter-1 and insulin receptor alpha1 in osteoblast obtained from diabetic rats' mandibles.
- Author:
Xuan WU
1
;
Hongchen LIU
;
Lingling E
;
Zhenchun LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Diabetes Mellitus, Experimental; Glucose Transport Proteins, Facilitative; Mandible; Osteoblasts; Rats; Receptor, Insulin
- From: West China Journal of Stomatology 2011;29(4):348-354
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression of glucose transporter (GLUT)-1 and insulin receptor (IR) alpha1 in osteoblast obtained from diabetic rats' mandibles.
METHODSThe expression of GLUT-1 and IR alpha1 of diabetic and control groups were measured by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry stain.
RESULTSThe mRNA expressions of GLUT-1 and IR alpha1 of diabetic group were significantly higher than control group (P<0.05). The protein expressions of GLUT-1 and IR alpha1 were similar to the mRNA expressions.
CONCLUSIONOsteoblasts obtained from diabetic rats' mandibles keep the adaptation changes to hyperglycemia and hypoinsulinemia, which may contribute to their dysfunction.
