A comparison of the myogenic characteristics between stem cell antigen-(1+) and stem cell antigen-(1-) cells derived from skeletal muscle.
- Author:
Xiaoxia CHE
1
;
Tong ZHAO
;
Lingling ZHU
;
Jie GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Ly; Cell Differentiation; Cell Proliferation; Cells, Cultured; Flow Cytometry; Membrane Proteins; Mice; Muscle, Skeletal; Myogenin; Stem Cells
- From: West China Journal of Stomatology 2011;29(4):369-374
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo probe into the function of stem cell antigen-1 (Sca-1) in cell proliferation and differentiation.
METHODSSca-(1+) and Sca-(1-) muscle derived cells were separated from C57BU6 mice by fluorescence-activated cell sorting and then cultured in vitro. After 5 days cells proliferative curve were drawn according CCK-8 experimental results. Western blot also were done to detect Sca(-1), MyoD and Myogenin expression in cultured Sca(-1+) and Sca-(1-) muscle derived cells.
RESULTSThe difference of the proliferative curve of Sca-(1+) and Sca-(1-) muscle derived cells cultured 3 days in vitro was not apparent, but Sca-(1-) muscle derived cells had a accelerated division rate in the follow days compared to the Sca-(1+) muscle derived cells. Sca-1 expression in both cells was not obvious. MyoD and Myogenin expression were stronger in Sca-(1+) than Sca-(1-) muscle derived cells.
CONCLUSIONSca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.
