Establishment of a green fluorescent protein-labeled mouse model of adenoid cystic carcinoma.
- Author:
Zheng-gang CHEN
1
;
Zuoqing DONG
;
Dong ZHANG
;
Chengjun ZHOU
;
Shanzhen SUN
;
Shaohua LIU
;
Fengcai WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Adenoid Cystic; Cell Line, Tumor; Cell Proliferation; Disease Models, Animal; Green Fluorescent Proteins; Humans; Mice; Mice, Inbred BALB C; Mice, Nude
- From: West China Journal of Stomatology 2011;29(4):424-428
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a novel nude mice model which can be visualized in real time and detected in a continuous and dynamic way for the development and metastasis of adenoid cystic carcinoma.
METHODSHuman adenoid cystic carcinoma cells, ACCM cell line, were infected with retroviral vector of pLEGFP-N1 and then screened for a single colony of ACCM-GFP cells. Cell proliferation and morphological analysis were conducted for ACCM and ACCM-GFP cells. Nude mice lingual carcinoma model was set up with ACCM-GFP cells injection and real time observation with fluorescence imaging on ACCM-GFP tumors was performed subsequently. Histological assay was analyzed for ACCM and ACCM-GFP tumors as well.
RESULTSACCM-GFP cells were able to express GFP stably in the long term. ACCM and ACCM-GFP cells showed no significant difference in cell proliferation and morphology, and no significant difference of histological characteristics in vivo could be found between ACCM and ACCM-GFP tumors. Tumor development could be monitored in real time with fluorescence imaging system in vivo.
CONCLUSIONGFP-expressing ACCM tumor model can be applied to detect and observe its development in the long term in a noninvasive, real time and dynamic way. It is also a kind of ideal in vivo mouse model for adenoid cystic carcinoma research.