An animal experiment of construction of functional tissue-engineered bone with cell sheet technology.
- Author:
Tao CHEN
1
;
Yanhui WANG
;
Lingxue BU
;
Ningyi LI
Author Information
- Publication Type:Journal Article
- MeSH: Animal Experimentation; Animals; Bone Marrow Cells; Bone and Bones; Cells, Cultured; Mesenchymal Stromal Cells; Osteoblasts; Osteogenesis; Platelet-Rich Plasma; Stromal Cells; Tissue Engineering
- From: West China Journal of Stomatology 2011;29(4):442-445
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct functional tissue-engineered bone with cell sheet technology and method of traditional bone tissue engineering.
METHODSCanine bone marrow mesenchymal stem cells (BMSCs) were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 degrees C, 5% CO2 and saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 degrees C. Demineralized bone matrix (DBM) and platelet-rich plasma (PRP) were prepared, and complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. Complex of DBM/PRP/BMSCs was implanted under the right latissimus dorsi muscle.
RESULTSWhen temperature dropped at 20 degrees C, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/ BMSCs group.
CONCLUSIONCell sheet technology combined with traditional bone tissue provides a new way for construction of ideal functional tissue-engineered bone.
