Genomics analysis of miRNA in colon cancer tissues.
- Author:
Xuehu XU
1
;
Xiaobing WU
;
Yong LI
;
Haibo LIU
;
Qinping JIANG
;
Yan SUN
Author Information
- Publication Type:Journal Article
- MeSH: Cluster Analysis; Colonic Neoplasms; genetics; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genomics; Humans; Lymphatic Metastasis; MicroRNAs; genetics
- From: Chinese Journal of Gastrointestinal Surgery 2014;17(11):1130-1132
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the differential expression of miRNAs in colon cancer tissues and matched tumor adjacent tissues.
METHODSDifferential expression of microRNAs in twenty paired human colon cancer tissues and adjacent tissues were detected by QPCR. miRNAs with significantly differential expression (fold change >2.4 and P<0.01) were screened to analyze their accumulation by Cluster analysis. Thus correlation of miRNA and other colon cancer-associated proteins was examined.
RESULTSExpressions of 17 miRNAs were significantly reduced in colon cancer tissues. Cluster analysis showed that miR763-3, miR451 and miR99a had similar expression. Plasma CK20 level was negatively correlated with miR100 (r=-0.948), miR152a-5p (r=-0.948), miR125b (r=-0.949), miR145 (r=-0.949) and miR145*(r=-0.949) (all P<0.05).
CONCLUSIONmiR145 and other 16 miRNAs may be used as diagnostic molecular markers of colon cancer, and miR100, miR125a-5p, miR125b, miR145 and miR145* may become the molecular markers of colon cancer lymph node metastasis.
