Expression of miR-143 and miR-145 and their functional study in gastric carcinoma.

Xiaofang Xing; Ziyu LI

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Expression of miR-143 and miR-145 and their functional study in gastric carcinoma.

Author: Xiaofang XING ¹ ; Ziyu LI
Author Information

1. Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education), Department of Gastrointestinal Cancer, Beijing Cancer Hospital and Institute, Peking University Cancer Hospital, Peking University School of Oncology, Beijing 100142, China. ligregory369@hotmail.com.
Publication Type:Journal Article
MeSH: Cell Movement; Gene Expression Regulation, Neoplastic; Humans; Liver Neoplasms; MicroRNAs; Stomach Neoplasms
From: Chinese Journal of Gastrointestinal Surgery 2015;18(1):50-53
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the expressions of miR-143 and miR-145 in gastric cancer tissues and their biological functions.
METHODSA set of gastric cancer metastasis-related miRNAs was screened by comparing the miRNA profiles between the primary gastric cancer and matched liver metastasis. Among these miRNAs, expressions of miR-143 and miR-145 were further validated. The influence on metastasis was analyzed by transwell assay.
RESULTSExpressions of miR-143 and miR-145 down-regulated significantly in gastric cancer tissues compared to adjacent normal tissues(miR-143, 0.028±0.005 vs. 0.052±0.014, P=0.058; miR-145, 0.922±0.135 vs. 1.722±0.285, P=0.007), and decreased significantly in the metastatic lesion compared with the primary lesion(miR-143, 0.059±0.025 vs. 0.182±0.045, P=0.021; miR-145, 0.164±0.076 vs. 0.594±0.283, P=0.042). Expressions of miR-143 and miR-145 were significantly correlated(r=0.400, P=0.000), and they synergistically inhibited gastric cancer cell migration.
CONCLUSIONmiR-143 and miR-145 may take part in the progression of gastric cancer metastasis, and they may have synergetic effects.