Experimental study of human bone marrow mesenchymal stem cells on regulating the biological characteristics of gastric cancer cells.
- Author:
Jia WANG
1
;
Jiwei YU
;
Jugang WU
;
Shoulian WANG
;
Dehu CHEN
;
Fan YANG
;
Bao HUA
;
Xiaolong XI
;
Shuzheng SONG
;
Linhai ZHENG
;
Bojian JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; Apoptosis; Bone Marrow Cells; Cadherins; Cell Line, Tumor; Cell Proliferation; Cisplatin; Coculture Techniques; Epithelial-Mesenchymal Transition; Fluorouracil; Humans; RNA, Messenger; Stem Cells; Stomach Neoplasms
- From: Chinese Journal of Gastrointestinal Surgery 2015;18(2):159-165
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulatory mechanism of bone marrow mesenchymal stem cells(BMSC) on the biological profiles of KATO-III( cell lines of gastric cancer.
METHODSTranswell cubicle was applied to build the co-cultured model in non-contact style. The differences of cell proliferation and the resistance of anti-tumour drug (5-fluoropyrimidinedione, 5-FU and Cisplatin, CDDP) between co-cultured group and single cultured group were evaluated by Cell Counting Kit 8-assay(CCK-8). The invasion ability was detected by Transwell assay. The expressions of stem cell makers, apoptosis-related factors and epithelium-mesenchymal transition (EMT)-related factors were detected by RT-PCR.
RESULTSThe proliferation ability of KATO-III( cells in co-cultured group was significantly stronger than that in single cultured group. The growth rate of KATO-III( cells in co-cultured group was significantly higher than that in single cultured group after treatment of 5-FU and CDDP(P<0.05). The mRNA expression level of Bcl-2 was significantly higher in co-cultured group KATO-III( cells(P<0.05), while the mRNA expression level of Bax was significantly lower in co-cultured group KATO-III( cells(P<0.05) in comparison with those in single cultured group. As compared to KATO-III( cells in single cultured group, the number of infiltrating-membrane cells was significantly higher (37.33±5.22 vs 14.56±2.54, P<0.01) in co-cultured group, and the mRNA expression levels of Snail and N-cadherin were significantly higher in co-cultured group KATO-III( cells (P<0.05), while the mRNA expression level of E-cadherin was significantly lower in co-cultured group KATO-III( cells (P<0.05). The expressions of CD133, Nanog and Sox-2 mRNA in co-cultured group KATO-III( cells were significantly higher than those in single cultured group(P<0.05).
CONCLUSIONSIn co-cultured model sharing non-contact style, BMSC can enhance such properties of KATO-III( gastric cancer cells as the proliferation, the invasion and the chemoresistance. Furthermore, the regulatory mechanisms may be related to the increase of the expressions of some stem cell markers in gastric cancer cells.
