Expression of prostaglandin transporter in colorectal cancer tissues and its relationship with clinicopathological features.
- Author:
Shanliang SHANG
1
;
Xiujun LIAO
;
Zhong SHEN
;
Jianming QIU
;
Shuxian SHAO
;
Lie DING
;
Dong WANG
;
Guangen YANG
;
Yanxiang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Colorectal Neoplasms; Down-Regulation; Humans; Neoplasm Invasiveness; Neoplasm Staging; Organic Anion Transporters; RNA, Messenger
- From: Chinese Journal of Gastrointestinal Surgery 2015;18(3):277-281
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of prostaglandin transporter (PGT) in colorectal cancer (CRC) tissues and its relationship with clinicopathological features.
METHODSThe mRNA and protein levels of PGT were determined by real-time PCR, Western blot and immunohistochemical methods in cancer tissues and adjacent normal tissue from 80 patients with colorectal cancer and their relationship with clinicopathological features was analyzed.
RESULTSCompared with the adjacent normal tissue of colorectal cancer, the PGT mRNA relative expression (0.57 ± 0.33 vs. 2.33 ± 1.20) and the PGT protein expression in cancer tissues decreased significantly [PGT/GAPDH 0.45 ± 0.16 vs. 0.78 ± 0.23, integral A 718.7 ± 359.4 vs. 10412.0 ± 6423.3, average A 0.03 ± 0.01 vs. 0.12 ± 0.09, all P<0.01]. Lower mRNA and protein expressions of PGT in colorectal cancer were associated with depth of invasion T3 to T4 and TNM stage III( to IIII( (P<0.01), while not associated with gender, age, tumor location and differentiation degree (all P>0.05).
CONCLUSIONExpression levels of PGT mRNA and protein in colorectal cancer tissue are significantly down-regulation. PGT expression is associated with invasion depth and late stages.
