Study on inhibitory effect of matrine on cyclooxygenase-2 expression in colon cancer HT-29 cell line.
- Author:
Jian HUANG
1
;
Ming-jie ZHANG
;
Fu-ming QIU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Cyclooxygenase 2; Dinoprostone; biosynthesis; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation, Enzymologic; HT29 Cells; Humans; Membrane Proteins; Prostaglandin-Endoperoxide Synthases; biosynthesis; genetics; Quinolizines; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(3):240-243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of matrine on cyclooxygenase-2 (COX-2) expression in colon cancer HT-29 cell line at the level of gene and protein.
METHODSLevels of mRNA and protein expression of COX-2, and its synthesized product prostaglandin E2 (PGE2) of colon cancer HT-29 cell line were detected by RT-PCR, Western-blot, ELISA respectively before and after treatment of matrine in different concentrations.
RESULTSMatrine had inhibitory effect on the mRNA and protein expression of COX-2, and synthesis of PGE2 in colon cancer HT-29 cell line, but had no effect on COX-1. When HT-29 cell line was treated with 2.0 mg/ml of matrine, the inhibitory rate on COX-2 mRNA expression were 100% at 6 hrs and 9 hrs after treatment; the inhibitory rate on PGE2 synthesis was 63.8 % at 9 hrs after treatment; and that on COX-2 protein expression was 48% and 100% 12 hrs and 24 hrs after treatment, respectively.
CONCLUSIONMatrine has selective inhibitory effect on gene transcription, protein expression and functional activity of COX-2 in HT-29 cell line, which is time-dependent and concentration-dependent within certain range of concentration and acting time.
