Effect of danshensu and ligustrazine on related genes of myocardial hypertrophy induced by angiotensin II.
- Author:
Zi-Qiang GUO
1
;
Shuo-Ren WANG
;
Ling-Qun ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin II; Animals; Animals, Newborn; Atrial Natriuretic Factor; biosynthesis; genetics; Cardiomegaly; chemically induced; metabolism; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Female; Lactates; pharmacology; Male; Myocytes, Cardiac; cytology; metabolism; Pyrazines; pharmacology; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Wistar
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(4):342-344
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Danshensu (DSS) and Ligustrazine (TMZ), the extracts of Chinese herbs for promoting blood circulation, on angiotensin II (Ang II) induced myocardial hypertrophy and its related genes, and to explore the mechanisms of inhibitory effect.
METHODSAdopting one-step method, the total RNA of myocardial cells was extracted by TRIzol reagent. Then the expression of ANP and beta-actin mRNA, as symbol of myocardial cells, were detected by RT-PCR.
RESULTSMolecular biological research showed that Ang II could significantly increase the expression of ANP mRNA in myocardial cells (P < 0.01), which could be significantly inhibited by Losartan (P < 0.01), both DSS and TMZ had the inhibitory effect (P < 0.05). Ang II could increase beta-actin mRNA expression in myocardial cells simultaneously, Losartan, DSS and TMZ could also significantly inhibit it (P < 0.05).
CONCLUSIONThe effective ingredients of Chinese herbs for promoting blood circulation, DSS and TMZ, have the effect of inhibiting the hyper-expression of ANP and beta-actin induced by Ang II, and preventing myocardial hypertrophy, therefore, it could be used to prevent and treat cardiomegaly.
