Effect of Bushen recipe on T-cell subsets and their function in patients with chronic hepatitis B.
- Author:
Yue-qiu GAO
1
;
Xue-hua SUN
;
Xiao-ying ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Drugs, Chinese Herbal; therapeutic use; Female; Hepatitis B e Antigens; blood; Hepatitis B virus; isolation & purification; Hepatitis B, Chronic; drug therapy; immunology; Humans; Interleukin-10; blood; Male; Middle Aged; Phytotherapy; T-Lymphocyte Subsets; immunology; Th1 Cells; immunology; Th2 Cells; immunology; Virus Replication; drug effects
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(5):408-411
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the regulatory effect of Bushen recipe (BSR) on immune function in patients with chronic hepatitis B.
METHODSThirty patients with HBeAg+ and abnormal alanine transaminase (ALT) level were treated with BSR for 6 months. The following parameters before treatment, 3 months and 6 months after treatment were tested: (1) HBV DNA by fluorescence quanitiative PCR; (2) Levels of interleukin-10 (IL-10) and interferon-gamma in supernatant of cultured peripheral blood mononuclear cell (PBMC) after had been cultured with HBeAg, HBcAg and phytohemagglutinin (PHA) for 48 hrs; (3) Blood levels of T-cells, including CD4+ , CD8+ , CD8+ CD28+ , CD8+ CD28- and CD28+ , and their ratio, as well as the percentage of CD8+ CD28+ in the cell culture after being co-cultured with PHA for 48 hrs.
RESULTSIn patients effectively treated by BSR, after 3 months treatment, level of IFN-gamma in PBMC culture significantly increased, CD8+ CD28+ , CD28+ T-cells significantly enhanced and IL-10 and CD8 + CD28+ T-cells significantly decreased (P < 0.05, P < 0.01).
CONCLUSIONBSR could significantly strengthen the expression of Th1 type cytokine, decrease the expression of Th2 type cytokine and promote the expression of CD8+ cytotoxic T-lymphocyte, which may be one of mechanisms in suppressing HBV DNA replication.
