Protective mechanisms of radix salviae miltiorrhizae against chronic alcoholic liver injury in mice.
- Author:
Zong-bin XIONG
1
;
Ping WU
;
Yun-feng HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; therapeutic use; Female; Heme Oxygenase (Decyclizing); biosynthesis; genetics; Heme Oxygenase-1; Hepatitis, Alcoholic; drug therapy; pathology; Liver; metabolism; Male; Membrane Glycoproteins; biosynthesis; genetics; Membrane Proteins; Mice; Phytotherapy; RNA, Messenger; biosynthesis; genetics; Receptors, Cell Surface; biosynthesis; genetics; Salvia miltiorrhiza; Toll-Like Receptor 4; Toll-Like Receptors
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(5):425-428
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective mechanisms of Radix Salviae miltiorrhizae (RSM) on chronic alcoholic liver injury in mice.
METHODSThe chronic alcoholic liver injury mouse model was established. The morphologic change of hepatic tissue was observed with hematoxylin-eosin (HE) staining; the levels of toll-like receptor-4 (TLR-4) mRNA in hepatic tissue and hemeoxygenase-1 (HO-1) mRNA were determined using reverse transcription polymerase chain reaction (RT-PCR) technique; and the expression of TLR-4 protein was determined by immunohistochemistry method.
RESULTSRSM could alleviate the fatty degeneration and adiponecrosis of hepatic cells induced by alcohol, down-regulate the expressions of TLR-4 mRNA and HO-1 mRNA, and significantly decrease the number of TLR-4 positive cells.
CONCLUSIONRSM could prevent liver injury from alcohol by way of influencing TLR-4 signal transcription.
