Inhibitory effect of active fraction of tiaoxin recipe on beta-amyloid protein induced long-term potentiation in CA1 area of rats' hippocampal slices.
- Author:
Hai-fa QIAO
1
;
Sheng YANG
;
Wen-xia ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Alzheimer Disease; drug therapy; physiopathology; Amyloid beta-Peptides; antagonists & inhibitors; Animals; Drugs, Chinese Herbal; pharmacology; Female; Hippocampus; physiopathology; Long-Term Potentiation; drug effects; Phytotherapy; Rats; Rats, Wistar
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(5):429-431
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of the active fraction of Tiaoxin recipe (TXR-A), in inhibiting long-term potentiation (LTP) induced by beta amyloid protein (beta-AP) in CA1 area of rats' hippocampal slices.
METHODSThe population spike (PS) in CA1 area of hippocampal slices incubated in different medium was recorded before and after LTP was evoked by a 100 Hz, 100 trains high frequency stimulation (HFS), using extracellular microelectrode recording techniques.
RESULTSThe amplitude of PS significantly decreased after HFS in hippocampal slices incubated in medium containing 0.2 micromol/L beta-AP for more than 1.5 hour, as compared with that incubated in normal cerebrospinal fluid, the difference was significant, suggesting that beta-AP could inhibit LTP in hippocampal slices. The average amplitude of PS in slices incubated in beta-AP containing medium could be significantly enhanced by adding high-concentration TXR-A or TXR into the medium, and TXR-A showed a better effect of enhancing than that of TXR, indicating that TXR-A could increase the amplitude of LTP.
CONCLUSIONTXR-A may be the chief ingredient extracted from TXR for improving beta-AP induced LTP in CA1 area of rats' hippocampus, to antagonise the inhibition of beta-AP on LTP is possibly one of the mechanisms for its intelligence benefiting action.
