Effect of curcumin on expression of human low density lipoprotein receptors in Xenopus Laevis oocytes.
- Author:
Chun-lei FAN
1
;
Xing-de WO
;
Yan LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Curcumin; pharmacology; Dose-Response Relationship, Drug; Female; Gene Expression Regulation; Humans; Microinjections; Oocytes; cytology; metabolism; Receptors, LDL; biosynthesis; genetics; Xenopus laevis
- From: Chinese Journal of Integrated Traditional and Western Medicine 2005;25(5):432-435
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the molecular mechanism of curcumin in reducing blood lipids by establishing gene expression system of human low density lipoprotein receptors (LDL-R) in Xenopus Laevis oocytes (XLO).
METHODSThe expression of LDL-R on cytomembrane was determined using immuno-fluorescent, ligand-fluorescent and immune colloidal gold techniques after human LDL-R containing p3.7 LDL plasmid was led into nucleus. And the expression of LDL-R gene in XLO was quantitatively determined by ELISA after being interfered with different concentrations of curcumin.
RESULTSThe human LDL-R gene could be expressed on XLO, which could be significantly enhanced by curcumin in a dose-dependent manner. Conclusion One of the paths of curcumin in reducing blood lipids and anti-atherosclerosis was improving LDL-R gene expression and increasing the LDL-cholesterol absorption of cells.
