Experimental study on effect of Xuefu Zhuyu Decoction on bone marrow hematopoietic stem cells of mice.
- Author:
Dong GAO
1
;
Jiu-Mao LIN
;
Liang-Pu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, CD34; biosynthesis; Antigens, Ly; biosynthesis; Bone Marrow Cells; cytology; drug effects; metabolism; Cell Count; Cells, Cultured; Colony-Forming Units Assay; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; pharmacology; Female; Hematopoietic Stem Cells; cytology; drug effects; metabolism; Male; Membrane Proteins; biosynthesis; Mice; Random Allocation
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(6):527-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Xuefu Zhuyu Decoction (XFZYD) on the number, phenotype, cell cycle and colony formation of bone marrow hematopoietic stem cells (HSC) in mice.
METHODSKunming mice were randomly divided into 4 groups: the control group, the low- (3.25 g/kg), middle- (6.5 g/kg) and high-dose (13.0 g/kg) XFZYD groups. After they were medicated by gastrogavage respectively with saline or corresponding dose of XFZYD for 7 days, their bone marrow HSC were separated and counted. The phenotype Sca and cell cycle of HSC were detected by flow cytometer, and the colony formation was determined with semisolid methyl media culture.
RESULTSNo obvious difference in the number of mononuclear cell, suspended cell and colony production was found among all the groups (P > 0.05); while the expression of CD34 and Sca-1 increased in the low-dose XFZYD group, but in the middle-dose XFZYD group increase only showed in Sca-1 expression.
CONCLUSIONXFZYD plays a role of removing blood stasis and promoting regeneration through improving hematopoietic function by means of increasing the number and enhancing the function of premature HSC.
