The high dosage of earthworm (Eisenia andrei) extract decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus.
10.5115/acb.2011.44.3.218
- Author:
Bing Chun YAN
1
;
Ki Yeon YOO
;
Joon Ha PARK
;
Choong Hyun LEE
;
Jung Hoon CHOI
;
Moo Ho WON
Author Information
1. Department of Neurobiology, Kangwon National University School of Medicine, Chuncheon, Korea. mhwon@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Neurogenesis;
Subgranular zone;
Eisenia andrei extract;
Neurotrophic factor
- MeSH:
Animals;
Brain-Derived Neurotrophic Factor;
Bromodeoxyuridine;
Cell Proliferation;
Dentate Gyrus;
Immunohistochemistry;
Mice;
Neurogenesis;
Oligochaeta
- From:Anatomy & Cell Biology
2011;44(3):218-225
- CountryRepublic of Korea
- Language:English
-
Abstract:
Earthworm extract has shown anticancer characteristics. In the present study, we examined the effect of chronic treatment with a high dose of earthworm (Eisenia andrei) extract (EE) on cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus (DG) of 3-week-old mice using 5-bromo-2'-deoxyuridine (BrdU) and Ki-67 immunohistochemistry for cell proliferation and doublecortin (DCX) immunohistochemistry for neuroblast differentiation, respectively. BrdU-, Ki-67-, and DCX-immunoreactive cells were easily detected in the subgranular zone of the DG in vehicle (saline)-treated mice. However, BrdU-, Ki-67-, and DCX-immunoreactive cells in the 500 mg/kg EE-treated mice decreased distinctively compared to those in the vehicle-treated mice. In addition, brain-derived neurotrophic factor (BDNF) immunoreactivity and its protein level decreased markedly in the DG of the EE-treated group compared to those in the vehicle-treated group. These results indicate that chronic treatment with high dose EE decreased cell proliferation and neuroblast differentiation, and that BDNF immunoreactivity decreased in the DG of EE-treated mice.
