Effects of TCM treatment according to syndrome differentiation on expressions of nuclear factor-kappaB and gamma-glutamylcysteine synthetase in rats with chronic obstructive pulmonary disease of various syndrome types.
- Author:
Wei ZHANG
1
;
Xin-Yue ZHANG
;
Yu-Meng SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Diagnosis, Differential; Drugs, Chinese Herbal; therapeutic use; Glutamate-Cysteine Ligase; biosynthesis; Immunohistochemistry; Male; Medicine, Chinese Traditional; NF-kappa B; biosynthesis; Phytotherapy; Pulmonary Disease, Chronic Obstructive; diagnosis; drug therapy; metabolism; Random Allocation; Rats; Rats, Wistar; Syndrome
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(5):426-430
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of traditional Chinese medicine (TCM) treatment according to syndrome differentiation in treating chronic obstructive pulmonary disease (COPD) by observing the changes of nuclear factor-kappaB (NF-kappaB) and gamma-glutamylcysteine synthetase (gamma-GCS) expression levels in rats.
METHODSCOPD model was established by modified method of combining fumigation and lipopolysaccharide (LPS) intra-tracheal dripping. Model rats were treated respectively for succesive 14 days according to their syndrome, that is, Xiaoqinglong Decoction to the rats of cold-phlegm accumulation in Fei, Maxing Shigan Decoction to those of heat-phlegm accumulation in Fei, Yupingfeng Decoction to those of Fei-qi deficiency, Liujunzi Decoction to those of Pi-qi deficiency, Renshen Gejie Decoction to those of Shen qi-deficiency. Besides, model rats in the model control group received 2mL normal saline daily, and no intervention was applied in the normal control group. The expression of gamma-GCS and NF-kappaB was detected by immunochemistry before and after treatment.
RESULTSCompared with that in the normal rats, the expressions of gamma-GCS and NF-kappaB in bronchial and alveolar epithelium of COPD rats before treatment were significantly higher, but the positive expression rates were lowered after treatment significantly (P<0.05).
CONCLUSIONTCM treatment according to syndrome differentiation could rectify imbalance of oxidation/anti-oxidation and alleviate inflammatory reaction in COPD rats, thus to treat COPD effectively.
