Effects of garlicin on NIH3T3 cell proliferation and collagen synthesis.
- Author:
Hai-Xiao ZHANG
1
;
Zai-Xiang SHI
;
Hai-Zhong JIA
Author Information
- Publication Type:Journal Article
- MeSH: Allyl Compounds; pharmacology; Animals; Cell Proliferation; drug effects; Collagen Type I; biosynthesis; Disulfides; pharmacology; Dose-Response Relationship, Drug; Garlic; chemistry; Hydroxyproline; analysis; Mice; NIH 3T3 Cells
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(5):431-434
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of garlicin on fibroblasts proliferation and type I collagen synthesis and explore its anti-fibrosis mechanism.
METHODSGarlicin was added into the culture fluid of NIH3T3 cell, taking Radix Salviae miltiorrhizae as the control medicine. The spiking of H3-thymidine DNA was detected, also the hydroxyproline (HOP) concentration in the culture fluid by alkali digestion method and the protein expression of type I collagen in NIH3T3 cells by immunofluorescent staining.
RESULTSThe NIH3T3 cell growth and proliferation rate were obviously reduced after garlicin treatment concentration-dependently in range of 0.2 - 5 microg/mL; HOP level and protein expression of type I collagen also lowered.
CONCLUSIONGarlicin could inhibit NIH3T3 cell proliferation, reduce the synthesis and protein expression of type I collagen so as to exert the anti-fibrosis effect.
