Effect of curcumin on activity of matrix metalloproteinase 2, 9 and nuclear expression of RelA in rat hepatic stellate cells by activating peroxisome proliferator-activated receptor gamma signal.
- Author:
Yang CHENG
1
;
Jian PING
;
Cheng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Active Transport, Cell Nucleus; drug effects; Animals; Blotting, Western; Cell Nucleus; drug effects; metabolism; Curcumin; pharmacology; Hepatic Stellate Cells; cytology; drug effects; metabolism; Male; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 9; metabolism; PPAR gamma; metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction; drug effects; Transcription Factor RelA; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(5):439-443
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of curcumin on the activity of matrix metalloproteinases (MMPs) and nuclear expression of RelA in rat hepatic stellate cells (HSCs) by activating peroxisome proliferator-activated receptor gamma (PPARgamma) signal in vitro.
METHODSHSCs were isolated from SD rats through in situ perfusion of liver, digestion with pronase E and density-gradient centrifugation with Nycodenz. The lytic HSCs were collected after treatment to extract the total protein and nucleoprotein for detecting the expression of PPARgamma, alphaSMA, collagen type I and RelA by Western blot, and the supernatant was collected to measure the activity of MMP2 and MMP 9 by gelatin zymograph method.
RESULTSThe PPARgamma expression decreased gradually with increasing of HSC activation, which was up-regulated by curcumin (P < 0.01); curcumin inhibited the expression of aSMA, the production of collagen type I, and the nuclear expression of activated RelA (P < 0.01), and elevated the activity of MMP2 and MMP9 significantly (P < 0.01). However, these effects were weakened by the PPARgamma antagonist, GW9662, significantly (P < 0.01).
CONCLUSIONBy activating PPARgamma signal transduction pathway curcumin treatment can inhibit HSC activation, increase the activity of MMP2 and MMP9 and inhibit/ interfere nuclear translocation of NFkappaB.
