Effects of tetramethylpyrazine on brain oxidative damage induced by intracerebral perfusion of L-DOPA in rats with Parkinson's disease.
- Author:
Dan-qiao WANG
1
;
Wei WANG
;
Fu-chun JING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents, Non-Steroidal; therapeutic use; Catechols; metabolism; Chromatography, High Pressure Liquid; methods; Corpus Striatum; drug effects; metabolism; pathology; Hydroxybenzoates; Levodopa; administration & dosage; Male; Microdialysis; Oxidative Stress; drug effects; Oxidopamine; Parkinson Disease, Secondary; chemically induced; drug therapy; metabolism; Pyrazines; therapeutic use; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(7):629-632
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of tetramethylpyrazine (TMP) on brain oxidative damage induced by intracerebral perfusion of levodopa (L-DOPA) in rats with Parkinson's disease (PD).
METHODSPD model rats were induced by intracerebral injection of 6-hydroxyl dopamine (6-OHDA) and perfused in brain with L-DOPA using microdialysis technique. Changes in levels of 2,3-dihydroxy benzyl acid (2.3-DHBA) and 2,5-dihydroxy benzyl acid (2,5-DHBA) in striatum of rats, formed by extracellular hydroxyl radical from salicylic acid capturing, were dynamically observed at various time points by HPLC-ED.
RESULTSAfter treatment with L-DOPA, 2,3-DHBA and 2,5-DHBA in the model group showed significantly higher levels at 6 and 7 time points as compared with those in the sham-operated group at the corresponding time points (P <0.05 or P< 0.01), while these abnormal elevations were significantly inhibited in the TMP treated groups, either in large or small dose (P<0.05 or P<0.01).
CONCLUSIONTMP could reduce the L-DOPA induced brain oxidative damage in PD rats.
