Effect of tanshinone II A on angiotensin II induced nitric oxide production and endothelial nitric oxide synthase gene expression in cultured porcine aortic endothelial cells.
- Author:
Yong-sheng LI
1
;
Qian-sheng LIANG
;
Jin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin II; pharmacology; Animals; Aorta; cytology; Cells, Cultured; Diterpenes, Abietane; Drugs, Chinese Herbal; pharmacology; Endothelial Cells; cytology; drug effects; metabolism; Gene Expression Regulation, Enzymologic; drug effects; Immunohistochemistry; Nitric Oxide; biosynthesis; Nitric Oxide Synthase Type III; biosynthesis; genetics; Phenanthrenes; pharmacology; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Swine
- From: Chinese Journal of Integrated Traditional and Western Medicine 2007;27(7):637-639
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of tanshinone II A on porcine aortic endothelial cells (PAEC).
METHODSPAEC were stimulated with angiotensin II (Ang- II) for different acting time (1 h, 6 h and 24 h) and Tanshinone II A was added along with Ang- II stimulation (Group A) or 6 h after it (Group B). The nitric oxide (NO) level, the protein and mRNA expression of nitric oxide synthase (cNOS) in PAEC were measured by nitric acid deoxidizing assay, RT-PCR and immunohistochemical assay, respectively.
RESULTSWith the prolongation of acting time of Ang- II, the level of NO and eNOS expression in PAEC sequentially decreased in a negative acting time dependent manner (P < 0.01), which could be inhibited by tanshinone II A treatment independent to the dosage used (P< 0.01). The inhibitory effect of tanshinone II A was better in Group A than that in Group B either at 1 h or at 6 h after treatment (P<0.05). However, 24 h later, no significant difference was found between the effect in the two groups (P >0.05).
CONCLUSIONTanshinone II A could inhibit the negative effect of Ang- II on NO production and eNOS expression in PAEC.
