Role of focal adhesion kinase in adhesion and migration of Hep G2 cells.
- Author:
Yunlong MA
1
;
Yang SHEN
;
Hongyi REN
;
Heng SUN
;
Hongchi YU
;
Xiaoheng LIN
Author Information
1. Institute of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Cell Adhesion;
drug effects;
Cell Movement;
drug effects;
Focal Adhesion Protein-Tyrosine Kinases;
antagonists & inhibitors;
metabolism;
physiology;
Hep G2 Cells;
Humans;
Liver Neoplasms;
pathology;
Neoplasm Metastasis;
prevention & control
- From:
Journal of Biomedical Engineering
2013;30(2):342-354
- CountryChina
- Language:Chinese
-
Abstract:
Focal adhesion kinase (FAK) plays a critical role in the process of cell adhesion and migration by regulating the expression of downstream small G proteins. A kind of focal adhesion kinase (FAK) inhibitor was used to inhibit the phosphorylation of Y397 site of FAK, and scratch wound migration assay was used to examine the effect of FAK inhibitor with different concentrations (0-250 nmol/mL) on the migration of hepatomal cells (Hep G2 cells) at 0, 2, 4, 8 and 24h. Immunofluorescence analysis and Western blot analysis were performed to detect the expression of F-actin and small G proteins Rac1, RhoA and Cdc42 in Hep G2 cells treated with FAK inhibitor for 120 min. The results indicated that the FAK inhibitor can inhibit the migration of Hep G2 cells with a dose- and time-dependent manner. F-actin was down-regulated in Hep G2 cells treated with FAK inhibitor for 120 min, and expression of small G proteins were inhibited at different durations. The inhibition of FAK phosphorylation could inhibit cell adhesion and migration by down-regulating small G proteins. These results suggested that FAK inhibitor can inhibit the migration of tumor cells by blocking FAK phosphorylation. This means that FAK inhibitor can block the metastasis of tumor cells to surrounding tissues. It may be a potential application in the prevention and treatment of cancer.
