S100 and p65 expression are increased in the masseter muscle after botulinum toxin-A injection.
10.1186/s40902-016-0079-8
- Author:
Young Wook PARK
1
;
Seong Gon KIM
;
You Young JO
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung 210-702, South Korea. kimsg@gwnu.ac.kr
- Publication Type:Original Article
- Keywords:
Botulinum toxin-A;
p65;
S100;
Apoptosis;
Masseter muscle
- MeSH:
Animals;
Apoptosis;
Biopsy;
Masseter Muscle*;
Muscle Cells;
Muscular Atrophy;
Myoblasts;
Myofibrils;
Rats
- From:Maxillofacial Plastic and Reconstructive Surgery
2016;38(8):33-
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The purpose of this study was to compare the expression levels of p65 and S100 in the rat masseter muscle after the injection of different concentrations of botulinum toxin-A (BTX-A). METHODS: We injected either 5 or 10 U of BTX-A into both masseter muscle of rats. As a control group, the same volume of saline was injected. After 14 days, the animals were sacrificed. Subsequently, a biopsy and immunohistochemical staining of the samples were performed using a p65 or S100 antibody. RESULTS: The cross-sectional area of each myofibril was significantly reduced by BTX-A injection (P < 0.001). The expression of p65 and S100 increased significantly with increasing concentrations of BTX-A (P < 0.001). CONCLUSIONS: The injection of BTX-A into the masseter muscle induced muscle atrophy. Subsequently, p65 and S100 expression in myoblasts were increased for the protection of muscle cells.
