Effects of electrical stimulation on the differentiation of mesenchymal stem cells into cardiomyocyte-like cells.
- Author:
Zhongqin YAN
1
;
Gang YANG
;
Liao CUI
;
Xueling HE
;
Wei KUANG
;
Wenchao WU
;
Xiaojing LIU
;
Liang LI
Author Information
1. Institute of Biomedical Engineering, West China College of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cell Differentiation;
Cell Proliferation;
Cells, Cultured;
Electric Stimulation;
Female;
Male;
Mesenchymal Stromal Cells;
cytology;
Myocytes, Cardiac;
cytology;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Biomedical Engineering
2013;30(3):556-561
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to investigate the effects of electrical stimulation ES) on the induction of rat bone marrow mesenchymal stem cells (rBMSCs) to differentiate into cardiomyocyte-like cells in vitro. The third or fourth-generation of the rBMSCs was randomly divided into three groups, i. e. ES group, 5-Azacytidine (5-Aza) group, and control group. Those in the ES group with complete medium were exposed to 1,2,4 and 6V, 2Hz, 5ms ES for 2h everyday,lasting for 10d. Those in the 5-Aza group were induced by 10 micromol/L 5-Aza for 24h, then the medium was changed to complete medium without 5-Aza. Those in the control group were only cultured with complete medium. The growth status and morphological features of rBMSCs were observed by inverted phase microscope. The mRNA expressions of GATA4, a-actin, ACTN2 and TNNT2 were determined by Real-time fluorescent quantification PCR, and the protein expression of TNNT2 was detected with immunofluorescence staining. The results showed that the mRNA expression level of the GATA4, a-actin, ACTN2 and TNNT2 and the protein expression level of the TNNT2 were significantly higher in the ES group and 5-Aza group, compared to those in the control group(P<0. 05). It suggested that ES could induce rBMSCs differentiation into cardiomyocyte-like cells in vitro.
