Characteristics of JC virus VLP-Z for targeting gene delivery.
- Author:
Junyan ZENG
1
;
Qiumin QU
Author Information
1. Department of Neurology, the First Affiliated Hospital of Medical College of Xi 'an Jiaotong University, Xi'an 710061, China.
- Publication Type:Journal Article
- MeSH:
Gene Targeting;
methods;
Gene Transfer Techniques;
Genetic Therapy;
methods;
Genetic Vectors;
genetics;
HeLa Cells;
Humans;
Immunoglobulin G;
metabolism;
JC Virus;
genetics;
metabolism;
Virion;
genetics;
metabolism
- From:
Journal of Biomedical Engineering
2013;30(3):572-576
- CountryChina
- Language:Chinese
-
Abstract:
The characteristics of virus-like particle (VLP) of JC virus (JCV) as a vector for targeting gene delivery was determined. The exogenous DNA (PUC19) packaged in VLP-Z was resistant to DNase I. VLP-Z was able to deliver a reporter plasmid pEGFP-N1 into HeLa cells and the green fluorescent reporter protein was expressed in these cells. VLP-Z was also able to bind IgG by interaction with the Z fragment of VLP-Z and IgG. These results suggested that VLP-Z might be used as a vector to deliver therapeutic genes to target cells with incorporating IgG antibodies.
