Differentiation of Bone Marrow Mesenchymal Cells to Neural Cells
- Author:
Yongchao WU
1
;
Qixin ZHENG
;
Xiaodong GUO
;
Zhongping XIE
;
Yuntao WANG
;
Jie HAO
Author Information
1. Union Hospital,Tongji Medical College,Huazhong University of Science and Technology
- Keywords:
bone mesenchymal stem cells;
cell differentiation;
neuron
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(5):488-490,493
- CountryChina
- Language:Chinese
-
Abstract:
To explore the possibility and condition of differentiation of bone marrow mesenchymal cells (BMSCs) to neural cells in vitro, BMSCs from whole bone marrow of rats were cultured. The BMSCs of passage 3 were identified with immunocytochemical staining of CD44 ( + ), CD71 ( + )and CD45(-). There were type Ⅰ and type Ⅱ cells in BMSCs. Type Ⅰ BMSCs were spindleshaped and strong positive in immunocytochemical staining of CD44 and CD71, whereas flat and big type Ⅱ BMSCs were lightly stained. The BMSCs of same passage were induced to differentiate into neural cells by β-mercaptoethanol (BME). After induction by BME, the type Ⅰ BMSCs withdrew to form neuron-like round soma and axon-like and dendrite-like processes, and were stained positively for neurofilament (NF). The type Ⅱ BMSCs did not change in the BME medium and were negatively or slightly stained of NF.
