IL-5 Up-regulates the Expression of TGF-β1 in Human Blood Eosinophils in Vitro
- Author:
Yabing HUANG
1
;
Bin LIU
;
Lu WANG
;
Rong LI
;
Min ZHU
;
Dong CHEN
;
Shi CHEN
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
IL-5;
transforming growth factor-beta1 (TGF-β1)
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(6):665-667
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of IL-5 on the expression of TGF-β1 in eosinophils in vitro, eosinophils were incubated in the presence of the same concentrations of IL-4, IL-5 and IFNγ, different concentrations of IL-5 in vitro and changes of eosinophil viability were assessed by trypan blue exclusion. Non-cytokine was employed as a negative control. 16 h after the cultivation, supernatants and cells were assayed by using TGF-β1 specific ELISA and RT-PCR. The mRNA expression and protein expresssion of TGF-β1 in eosinophils stimulated with different cytokines was observed.The expression of TGF-β1 protein in eosinophils was increased significantly by IL-4 (433.67±9.86vs 228.9±2.87) and IL-5 (403. 72±7.60 vs 228.9±2.87, P＜0.05), while decreased by IFNγ (178.47±2.60 vs 228.9±2.87). At the same time, the results demonstrated that the basal level of TGF expression was enhanced by IL5 in all samples (P＜0.05). The expression of TGF β1 mRNA was 1.42, 1. 70, 1. 76-folds higher than that of the non-stimulated controls. It is concluded that IL-5 can up-regulate the expression of TGF-β1 in eosinophils in vitro, which might have effect in eosinophil-associated chronic rejection.
