A Comparative Study of 99mTc-YIGSR and 99mTc-MIBI Uptake in Tumor Cells
- Author:
Jia HU
1
;
Xiaoli LAN
;
Yongxue ZHANG
;
Zairong GAO
;
Jun WU
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
laminin-YIGSR;
methoxyisobuty (MIBI-lisonitrile);
tumor;
cell uptake;
99mTc
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(6):712-714
- CountryChina
- Language:Chinese
-
Abstract:
To investigate a new kind of tumor tracer 99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin -chain,which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG3. (1) Preparation of the 99m Tc-YIGSR probe: with S-Acetly-NH3-MAG3as the chelator and with proper reductants YIGSR was labeled with 99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EACs uptake of 99mTc-YIGSR and99mTc-MIBI was observed at 37 ℃ and 22 ℃, respectively. (1)The labeling efficiencies of 99mTc-YIGSR and99mTc-MIBI were (62±3) % and (96±2) %, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 ℃, the EACS uptake of 99mTc-YIGSR and99mTc-MIBI reached the peak of (43. 16±2.4) % and (24.4±1.8) % at 60min, respectively; and at 22 ℃, the highest uptake was (26.5±2.1) % and (9. 47±1.9) % at 60min, respectively. The in vitro study suggests that 99mTc-YIGSR is superior to 99mTc-MIBI in cell uptake and has potential value in tumor imaging.
