To identify a novel HLA-DRB1 allele in Chinese by sequencing.
- Author:
Wei LI
1
;
Xiao-yan SHAN
;
Na LIU
;
Lei NI
;
Li-jun WANG
;
Lin WANG
;
Shuang CUI
;
Xiao-mei HE
;
Zhi-yin GONG
;
Bo-tao ZHAO
;
Zhi-xin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; genetics; HLA-DR Antigens; genetics; HLA-DRB1 Chains; Humans; Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2010;27(3):333-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify a novel HLA-DRB1 allele in Chinese.
METHODSA novel HLA-DR allele was detected by PCR-SSP and SBT in a patient with leukemia.
RESULTSThe sequence of the novel allele was different from all other known alleles. The novel allele differed from the closet matching allele HLA-DRB1*1404 by one nucleotide substitution in exon 2, at position 33 T>C, this resulted in an amino acid change from Tyr to His at codon 17.
CONCLUSIONThe novel allele is confirmed as a new HLA allele and it was officially named HLA-DRB1*1461 by WHO Nomenclature Committee in May, 2006.
