Application of fluorescence in situ hybridization technique in diagnosis of variant Ph chromosome translocation and Ph-negative chronic myelocytic leukemia.
- Author:
Yuan JING
1
;
Feng JIANG
;
Shuang LIN
;
Fang-ting WANG
;
Mei-yun FANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Chromosomes; genetics; Female; Fusion Proteins, bcr-abl; genetics; Humans; In Situ Hybridization, Fluorescence; methods; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; diagnosis; genetics; Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative; diagnosis; genetics; Male; Middle Aged; Philadelphia Chromosome; Translocation, Genetic; Young Adult
- From: Chinese Journal of Medical Genetics 2010;27(5):563-566
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the value of fluorescence in situ hybridization (FISH) technique in diagnosis of variant Ph chromosome translocation (VT) and Ph chromosome-negative chronic myelocytic leukemia (CML).
METHODSNine CML patients with VT and 2 Ph chromosome-negative CML patients confirmed by R banding were assayed with dual color-dual fusion BCR/ABL probe by FISH.
RESULTSThe 9 patients with VT involved chromosomes 1, 3, 5, 12, 13, 15, 17 and 21 besides chromosomes 9 and 22, and some of them showed recurrent aberrations; FISH results were positive and the signal feature was 2R2G1Y. The 2 Ph-negative CML patients had normal karyotypes; FISH was positive and the signal feature was 1R1G2Y and 1R1G1Y respectively.
CONCLUSIONFISH can provide better diagnosis for CML with VT and Ph-negative CML. Abnormal karyotype and marker gene changes can be assessed based on the signal feature of the positive cell. So FISH is a complementary method to banding technique in diagnosis of CML.
