Identification of the new allele D18S1364-10 by sequence analysis.
- VernacularTitle:序列分析鉴定新等位基因D18S1364-10
- Author:
Ying ZHAO
1
;
Wei-hua XU
;
Sheng-miao FU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alleles; Base Sequence; Child; Female; Humans; Microsatellite Repeats; genetics; Molecular Sequence Data; Sequence Analysis, DNA; methods
- From: Chinese Journal of Medical Genetics 2010;27(6):704-706
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify a new allele by analyzing the polymorphism of D18S1364 in Power Plex(TM)16.
METHODSAn abnormal band was found in paternity test by short tandem repeat-PCR, and collected by gel extraction. Then the DNA was amplified, cloned and sequenced.
RESULTSA fragment containing eight bases less than the minimal allele in the D18S1364 ladder, was found with the core sequence of (ATCT)₆(ATGT)₁(ATCT)₃. The allele was D18S1364-10.
CONCLUSIONThe D18S1364-10 allele was found and reported for the first time in China.
