Establishment of B lymphoblastoid cell lines of Miao pedigree with Bardet-Biedl syndrome.
- Author:
Tao SHEN
1
;
Tao SHOU
;
Ke-qin LIN
;
Wen YI
;
Ying-kun HUA
;
Hong DONG
;
Jian-mei GAO
;
Jin-ping ZHANG
;
Lin-ping WANG
;
Jin-li WANG
;
Li LI
;
Xin-min YAN
Author Information
- Publication Type:Journal Article
- MeSH: B-Lymphocytes; cytology; Bardet-Biedl Syndrome; blood; genetics; Cell Line; Cell Line, Transformed; Cell Transformation, Viral; China; ethnology; Ethnic Groups; genetics; Herpesvirus 4, Human; Humans; Pedigree
- From: Chinese Journal of Medical Genetics 2011;28(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish immortalized lymphoblastoid cell lines of a Miao core pedigree with Bardet-Biedl syndrome (BBS), in order to provide a long-term source of material for research.
METHODSWith Epstein-Barr virus transformation of B cells and addition of cyclosporine A to inhibit the activity of T cells, fresh anticoagulated blood samples with heparin were collected from 12 members of the core pedigree, and were used to establish the immortalized lymphoblastoid cell lines of B lymphocytes.
RESULTSTwelve immortalized lymphoblastoid cell lines of the core BBS pedigree were obtained successfully.
CONCLUSIONThe immortalized B lymphoblastoid cell lines of the Miao pedigree with BBS can preserve the whole genome information and provide long-term research materials for BBS study.
