Study on polymorphism of membrane glycoprotein genes related to human platelet alloantigens.

Author: Xiao-fei LAN ^{1
,

2} ; Yan-ling YING ; Ying LIU ; Xian-guo XU ; Fa-ming ZHU ; Hang-jun LV ; Li-xing YAN
Author Information

1. Institute of Transfusion Medicine, Blood Center of Zhejiang Province
2. Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou, Zhejiang, 310006 PR China.
Publication Type:Journal Article
MeSH: Antigens, Human Platelet; genetics; Humans; Isoantigens; genetics; Platelet Membrane Glycoproteins; genetics; Polymorphism, Single Nucleotide
From: Chinese Journal of Medical Genetics 2011;28(1):37-41
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the polymorphisms of platelet membrane glycoprotein genes related to human platelet alloantigen (HPA)-1 to 17w.
METHODSThe DNA segments of platelet membrane glycoprotein genes related to HPA-1 to 17w were amplified using author's designed primers. The amplification products were purified and directly sequenced to identify the HPA genotype and glycoprotein gene polymorphisms.
RESULTSThirteen new single nucleotide polymorphisms (SNPs) and a micro-satellite sequence were found in the glycoprotein genes from the 112 random samples, in which two SNPs (1333G/A and 1960G/A) in ITGB3 gene result in two amino acid change (V419M and E628K) on glycoprotein GPIIIa.
CONCLUSIONNew variants in platelet membrane glycoprotein genes were identified, which may lead to structure change of platelet membrane glycoprotein and affect the accuracy of partial HPA genotyping method.